NFkB (p50, p52, p65, c-Rel, RelB) Transcription Factor Assay Kit (Colorimetric) (ab207216)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Cell Lysate, Nuclear Extracts
- Sensitivity: 500 ng/well
Properties
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Storage instructions
Please refer to protocols. -
Components 2 x 96 tests 10X Antibody Binding Buffer 2 x 2.2ml 10X Wash Buffer 1 x 60ml 96-well NFkB assay plate 2 units Anti-rabbit HRP-conjugated IgG (0.25 μg/μL) 2 x 11µl Binding Buffer 1 x 10ml Developing Solution 2 x 11ml Dithiothreitol (DTT) (1 M) 1 x 100µl Herring sperm DNA (1 μg/μL) 1 x 100µl Lysis Buffer 1 x 10ml Mutated oligonucleotide (10 pmol/µL) 1 x 100µl NFkB c-Rel antibodies 1 x 11µl NFkB p50 antibodies 1 x 11µl NFkB p52 antibodies 1 x 11µl NFkB p65 antibody 1 x 11µl NFkB RelB antibodies 1 x 11µl Plate sealer 2 units Protease Inhibitor Cocktail 1 x 100µl Raji nuclear extract (2.5 µg/µL) 1 x 40µl Stop Solution 1 x 60ml Wild-type oligonucleotide (10 pmol/µL) 1 x 100µl -
Research areas
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Relevance
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. -
Cellular localization
Cytoplasmic and Nuclear. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). -
Alternative names
- MGC131774
- NFKB 3
- NFKB3
see all -
Database links
- Entrez Gene: 5970 Human
- Omim: 164014 Human
- SwissProt: Q04206 Human
- Unigene: 502875 Human
Images
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Nuclear extracts were assayed for NFkB activity using ab207216.
Nuclear extracts (10 µg/well) prepared a variety of cell lines were tested for activity of the different NFkB family members. Cell lines were untreated HeLa cells, HeLa cells treated with TNFα, untreated Jurkat cells, Jurkat cells treated with PMA and calcium ionophore (CI), and Raji cells. These results are provided for demonstration only.
