Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 40 min
Overview
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Product name
Neutrophil Elastase Inhibitor Assay Kit
See all Neutrophil Elastase kits -
Detection method
Fluorescent -
Assay type
Quantitative -
Assay time
0h 40m -
Product overview
In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
Visit our FAQs page for tips and troubleshooting. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Inhibitor Control (3 mM, SPCK) Purple 1 x 100µl Neutrophil Elastase Green 1 vial Substrate Red 1 x 0.2ml -
Research areas
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Relevance
Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage. -
Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
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Images
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Neutrophil Elastase Inhibitor Screening Kit (ab118971)
Neutrophil Elastase Activity Profile at Various Concentrations of Elafin/Skalp (ab153768)
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Neutrophil Elastase Inhibitor Screening Kit (ab118971)Neutrophil Elastase Activity Profile at Various Concentrations of SERPINB1 (ab114774)
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Functional Studies - Neutrophil Elastase Inhibitor Screening Kit (ab118971)Neutrophil Elastase Inhibition by SPCK -
Functional Studies - Neutrophil Elastase Inhibitor Screening Kit (ab118971)Neutrophil Elastase Activity Profile at Various Concentrations of SPCK
