Native human Neutrophil Elastase protein (Active) (ab91099)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
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Product name
Native human Neutrophil Elastase protein (Active)
See all Neutrophil Elastase proteins and peptides -
Biological activity
Activity: 20-22 units per mg protein.
One unit is defined as the amount of enzyme that hydrolyzes one micromole of Meo-suc-ala-pro-val-pNA per minute at 25°C in 100 mM Tris-HCl, pH 7.5, with 500 mM NaCl.
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Sequence
MTLGRRLACL FLACVLPALL LGGTALASEI VGGRRARPHA WPFMVSLQLR GGHFCGATLI APNFVMSAAH CVANVNVRAV RVVLGAHNLS RREPTRQVFA VQRIFENGYD PVNLLNDIVI LQLNGSATIN ANVQVAQLPA QGRRLGNGVQ CLAMGWGLLG RNRGIASVLQ ELNVTVVTSL CRRSNVCTLV RGRQAGVCFG DSGSPLVCNG LIHGIASFVR GGCASGLYPD AFAPVAQFVN WIDSIIQRSE DNPCPHPRDP DPASRTH -
Predicted molecular weight
30 kDa -
Amino acids
1 to 267
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 100% Elastase
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionAfter initial centrifugation, we suggest the addition of 50 mM Na Acetate, pH 5.5, with 150 mM NaCl to the original volume, followed by gentle swirling and/or vortexing to ensure adequate homogenization.
Images
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SDS-PAGE - Native human Neutrophil Elastase protein (ab91099)
12% Bis-Tris NuPAGE gel
Lane 1: 5 μg Neutrophil Elastase (reduced/heated)
Lane 2: 10 μg Neutrophil Elastase (reduced/heated)
Lane 3: 20 μg Neutrophil Elastase (reduced/heated)
Lane 4: Molecular weight markers -
Western blot - Native human Neutrophil Elastase protein (ab91099)Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml +Native human Neutrophil Elastase protein (Active) (ab91099) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
