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Signal Transduction Signaling Pathway Calcium Signaling Calpain

Native human Calpain 1 protein (Active) (ab91019)

Native human Calpain 1 protein (Active) (ab91019)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: WB, Functional Studies, SDS-PAGE

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Description

  • Product name

    Native human Calpain 1 protein (Active)
    See all Calpain 1 proteins and peptides
  • Biological activity

    >=1,000 units per mg protein.

    One unit is defined as the amount of enzyme that hydrolyzes one picomole of Suc-LLVY-AMC per minute at 25degC.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Accession

    P07384
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Predicted molecular weight

      110 kDa

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 6.80
    Preservative: 0.14% Imidazole
    Constituents: 0.038% EGTA, 0.039% Beta mercaptoethanol, 0.0292% EDTA, 30% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • SDS-PAGE - Native human Calpain 1 protein (ab91019)
    SDS-PAGE - Native human Calpain 1 protein (ab91019)

    SDS-PAGE: 4-12% Bis-Tris NuPAGE gel


    Lane 1: 5 μg ab91019 (reduced /heated). 
    Lane 2: 10 μg ab91019 (reduced/ heated). 
    Lane 3: Molecular weight markers.
    Lane 4: 5 μg ab91019 (non-reduced/no heat). 
    Lane 5: 10 μg ab91019 (non-reduced/no heat).

  • Western blot - Native human Calpain 1 protein (ab91019)
    Western blot - Native human Calpain 1 protein (ab91019)
    Anti-Calpain 1 antibody (ab2543) at 1 µg/ml + Native human Calpain 1 protein (Active) (ab91019) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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