N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540)
Key features and details
- Assay type: Competitive
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Purified protein, Serum
Overview
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Product name
N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell Lysate, Purified protein -
Assay type
Competitive -
Range
0.1 µg/ml - 25 µg/ml -
Product overview
N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540) is designed for the rapid detection and quantitation of CEL protein adducts.
First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the pre-determined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in this kit will not cross react with CML protein adducts.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 100X Conjugate Diluent 1 x 300µl 10X Wash Buffer 1 x 100ml Protein Binding Strip Well Plate 1 unit Anti-CEL Antibody (1000X) 1 x 10µl Assay Diluent 1 x 50ml CEL Conjugate 1 x 20µl CEL-BSA Standard 1 x 30µl Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl Stop Solution 1 x 12ml Substrate Solution 1 x 12ml
Images
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Example CEL-BSA ELISA Standard Curve.
Typical CEL ELISA results.
This data is for reference only and should not be used to interpret actual results.