Myo-D positive control ChIP primer pair (ab269261)
Key features and details
- Myo-D positive control ChIP primer pair
- Suitable for: ChIP
Overview
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Product name
Myo-D positive control ChIP primer pair
See all MyoD1 primary antibodies -
Description
Myo-D positive control ChIP primer pair -
Tested applications
Suitable for: ChIPmore details -
General notes
Positive control ChIP-qPCR 5' and 3' primers for Myo-D gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.
Suitable positive control for:
- Histone H3 tri methyl K27
- unmodified Histone H3
- Histone H3 mono methyl K4
- unmodified Histone H2B
- unmodified Histone H4
- Histone H3 mono methyl K9
- Histone H4 mono methyl K20
- unmodified Histone H2A500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
Properties
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Form
Lyophilized -
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading... -
Clonality
Monoclonal -
Research areas
Images
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ChIP - Myo-D positive control ChIP primer pair (ab269261)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab6002 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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ChIP - Myo-D positive control ChIP primer pair (ab269261)Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176880 (red), and 20µl of Protein A/G sepharose beads. Rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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ChIP - Myo-D positive control ChIP primer pair (ab269261)Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with 0.75% formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab176877 (blue), and 20μl of Anti-rabbit IgG agarose beads. Rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the γ-Actin gene (active). Schematic diagram of the γ-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
