Mouse XIAP ELISA Kit (ab254504)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 32 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse XIAP ELISA Kit
See all XIAP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 24 3.8% Inter-assay Sample n Mean SD CV% Extract 3 2.4% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
32 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 96 88% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse XIAP ELISA Kit (ab254504) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of XIAP protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse XIAP with 32 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
XIAP plays a key role in various cellular processes, such as the regulation of cell proliferation, apoptosis, inflammation, proteasomal regulation, and immunity. One of XIAP’s predominant roles is the blocking of apoptosis through binding and direct inhibition of caspases. Increased expression of XIAP is a hallmark of some cancers including those of the lung and prostate.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse XIAP Capture Antibody 1 x 600µl 10X Mouse XIAP Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse XIAP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. -
Tissue specificity
Ubiquitous, except peripheral blood leukocytes. -
Involvement in disease
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
Domain
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. -
Post-translational
modificationsUbiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AP 13
- API3
- Apoptosis Inhibitor 3
see all -
Database links
- Entrez Gene: 11798 Mouse
- SwissProt: Q60989 Mouse
- Unigene: 259879 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse XIAP standard curve in 1X Cell Extraction Buffer PTR.The XIAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native XIAP in NIH3T3 cell extract and mouse brain extract. The concentrations of XIAP were measured in duplicate and interpolated from the XIAP standard curve.The concentrations of XIAP were measured in duplicate and interpolated from the XIAP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean XIAP concentration was determined to be 2.51 ng/mL in NIH3T3 cell extract, and 1.70 ng/mL in mouse brain extract.
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Sandwich ELISA - Mouse XIAP ELISA Kit (ab254504)To learn more about the advantages of recombinant antibodies see here.
