Mouse VE Cadherin ELISA Kit (ab206980)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 167.6 pg/ml
- Range: 234.37 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse VE Cadherin ELISA Kit
See all VE Cadherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 4.7% Inter-assay Sample n Mean SD CV% Overall 3 6.6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
167.6 pg/ml -
Range
234.37 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 107% - 109% Tissue Culture Media 103 101% - 104% Hep Plasma 95 86% - 100% EDTA Plasma 99 94% - 103% Cit plasma 106 105% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse VE Cadherin ELISA Kit (ab206980) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VE Cadherin protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VE Cadherin with 167.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
VE-Cadherin (also known as Cadherin 5 or CD144) is a calcium-dependent cell-cell adhesion glycoprotein. VE-Cadherin is a member of the cadherin superfamily, which interact with themselves in connecting cells. VE-Cadherin is expressed in endothelial cells and interacts with KRIT1 to establish and maintain endothelial polarity.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse VE-Cadherin Capture Antibody 1 x 600µl 10X Mouse VE-Cadherin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse VE-Cadherin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
Tissue specificity
Endothelial tissues and brain. -
Sequence similarities
Contains 5 cadherin domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
Cellular localization
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Alternative names
- 7B 4
- 7B4
- 7B4 antigen
see all -
Database links
- Entrez Gene: 12562 Mouse
- SwissProt: P55284 Mouse
- Unigene: 21767 Mouse
Images
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Other - Mouse VE Cadherin ELISA Kit (ab206980)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of VE-Cadherin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution of native mouse VE-Cadherin protein in mouse serum and plasma.The concentrations of mouse VE-Cadherin were measured in duplicate and interpolated from the mouse VE-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). 1X serum is at a concentration of 0.25%, and 1X plasma (citrate and EDTA) is at a concentration of 0.5%, and 1X plasma (heparin) is at a concentration of 0.25%.
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Sandwich ELISA - Mouse VE Cadherin ELISA Kit (ab206980)To learn more about the advantages of recombinant antibodies see here.
