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Mouse VE Cadherin ELISA Kit (ab206980)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 167.6 pg/ml
  • Range: 234.37 pg/ml - 15000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse VE Cadherin ELISA Kit
    See all VE Cadherin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 6.6%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    167.6 pg/ml

  • Range

    234.37 pg/ml - 15000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 107% - 109%
    Tissue Culture Media 103 101% - 104%
    Hep Plasma 95 86% - 100%
    EDTA Plasma 99 94% - 103%
    Cit plasma 106 105% - 109%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse VE Cadherin ELISA Kit (ab206980) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VE Cadherin protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VE Cadherin with 167.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    VE-Cadherin (also known as Cadherin 5 or CD144) is a calcium-dependent cell-cell adhesion glycoprotein. VE-Cadherin is a member of the cadherin superfamily, which interact with themselves in connecting cells. VE-Cadherin is expressed in endothelial cells and interacts with KRIT1 to establish and maintain endothelial polarity.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse VE Cadherin ELISA Kit (ab206980)
    Other - Mouse VE Cadherin ELISA Kit (ab206980)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of VE-Cadherin standard curve.
    Example of VE-Cadherin standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Linearity of dilution of native mouse VE-Cadherin protein in mouse serum and plasma.
    Linearity of dilution of native mouse VE-Cadherin protein in mouse serum and plasma.

    The concentrations of mouse VE-Cadherin were measured in duplicate and interpolated from the mouse VE-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). 1X serum is at a concentration of 0.25%, and 1X plasma (citrate and EDTA) is at a concentration of 0.5%, and 1X plasma (heparin) is at a concentration of 0.25%.

  • Sandwich ELISA - Mouse VE Cadherin ELISA Kit (ab206980)
    Sandwich ELISA - Mouse VE Cadherin ELISA Kit (ab206980)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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