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Mouse TIMP2 ELISA Kit (ab227893)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 21 pg/ml
  • Range: 47 pg/ml - 3000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse TIMP2 ELISA Kit
    See all TIMP2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    21 pg/ml

  • Range

    47 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99 92% - 103%
    Cell culture media 118 116% - 119%
    Hep Plasma 103 102% - 105%
    Cit plasma 94 86% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse TIMP2 ELISA Kit (ab227893) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP2 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TIMP2 with 21 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse TIMP2 ELISA Kit (ab227893)
    Other - Mouse TIMP2 ELISA Kit (ab227893)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of Mouse TIMP2 standard curve in Sample Diluent LS
    Example of Mouse TIMP2 standard curve in Sample Diluent LS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native TIMP2 in Mouse serum and plasma samples
    Interpolated concentrations of native TIMP2 in Mouse serum and plasma samples

    The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (heparin) 1:200, and plasma (citrate) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 519 ng/mL in serum, 548 ng/mL in plasma (heparin) and 448 ng/mL in plasma (citrate).

  • Interpolated concentrations of native TIMP2 in Mouse cell culture supernatant samples
    Interpolated concentrations of native TIMP2 in Mouse cell culture supernatant samples

    The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleenocyte cell culture supernatant 25% and mouse lung cell culture supernatant 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 9.8 ng/mL in spleenocyte cell culture supernatant and 78.9 ng/mL in lung supernatant.

  • Sandwich ELISA - Mouse TIMP2 ELISA Kit (ab227893)
    Sandwich ELISA - Mouse TIMP2 ELISA Kit (ab227893)
    To learn more about the advantages of recombinant antibodies see here.

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