Mouse TIMP2 ELISA Kit (ab227893)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21 pg/ml
- Range: 47 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TIMP2 ELISA Kit
See all TIMP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3% Inter-assay Sample n Mean SD CV% Serum 3 5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
21 pg/ml -
Range
47 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 92% - 103% Cell culture media 118 116% - 119% Hep Plasma 103 102% - 105% Cit plasma 94 86% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse TIMP2 ELISA Kit (ab227893) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP2 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TIMP2 with 21 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent BAR 1 x 6ml 10X Mouse TIMP2 Capture Antibody 1 x 600µl Mouse TIMP2 Lyophilized Recombinant Protein 2 vials 10X MouseTIMP2 Detector Antibody 1 x 600µl Plate Seals 1 unit Sample Diluent LS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP2 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSC 21K
- CSC-21K
- CSC21K
see all -
Database links
- Entrez Gene: 21858 Mouse
- SwissProt: P25785 Mouse
- Unigene: 206505 Mouse
Images
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Other - Mouse TIMP2 ELISA Kit (ab227893)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Mouse TIMP2 standard curve in Sample Diluent LSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TIMP2 in Mouse serum and plasma samplesThe concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (heparin) 1:200, and plasma (citrate) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 519 ng/mL in serum, 548 ng/mL in plasma (heparin) and 448 ng/mL in plasma (citrate).
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Interpolated concentrations of native TIMP2 in Mouse cell culture supernatant samplesThe concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleenocyte cell culture supernatant 25% and mouse lung cell culture supernatant 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 9.8 ng/mL in spleenocyte cell culture supernatant and 78.9 ng/mL in lung supernatant.
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Sandwich ELISA - Mouse TIMP2 ELISA Kit (ab227893)To learn more about the advantages of recombinant antibodies see here.
