Mouse SET7 ELISA Kit (ab279404)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SET7 ELISA Kit
See all SET7 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.7% Inter-assay Sample n Mean SD CV% Extract 3 6.16% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 105 100% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse SET7 ELISA kit (ab279404) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse SET7 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SET7 with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
SET7, Histone-lysine N-methyltransferase SETD7, is a critical methyltransferase in epigenetic modification of histones. It specifically methylates lys4 of histone H3. It is homologous with many species throughout Mammalia, and as such is expected to cross-react with many species. The immunogen from which the antibodies in this kit were raised represents was a full-length recombinant protein with a minor N-terminal truncation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse SET7 Capture Antibody 1 x 600µl 10X Mouse SET7 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Mouse SET7 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro. -
Tissue specificity
Widely expressed. Expressed in pancreatic islets. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
Contains 3 MORN repeats.
Contains 1 SET domain. -
Domain
The SET domain is necessary but not sufficient for histone methyltransferase activity. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Alternative names
- FLJ21193
- H3 K4 HMTase
- H3-K4-HMTase SETD7
see all -
Database links
- Entrez Gene: 73251 Mouse
- SwissProt: Q8VHL1 Mouse
- Unigene: 192111 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of mouse SET7 standard curve in 1X Cell Extraction Buffer PTR.The SET7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SET7 in mouse NIH-3T3 cell extract based on a 100 µg/mL extract load.The concentrations of SET7 were measured in duplicate and interpolated from the SET7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SET7 concentration was determined to be 1,035 pg/mL.