Mouse Resistin ELISA kit (RETN) (ab108900)
Key features and details
- Sensitivity: 0.04 ng/ml
- Range: 0.063 ng/ml - 4 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Resistin ELISA kit (RETN)
See all Resistin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3.1% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.04 ng/ml -
Range
0.063 ng/ml - 4 ng/ml -
Recovery
97 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Resistin (RETN) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin levels in plasma, serum and cell culture supernatants.
A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse Resistin Antibody 1 x 140µl Chromogen Substrate 1 x 8ml Resistin Microplate (12 x 8 well strips) 1 unit Resistin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
Tissue specificity
Expressed only in fatty tissues. -
Sequence similarities
Belongs to the resistin/FIZZ family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
Database links
- Entrez Gene: 57264 Mouse
- SwissProt: Q99P87 Mouse
- Unigene: 1181 Mouse
Images
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Typical Standard Curve
Representative Standard Curve using ab108900
