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Mouse PTX3 ELISA Kit (ab245713)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 22.3 pg/ml
  • Range: 156.25 pg/ml - 10000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse PTX3 ELISA Kit
    See all Pentraxin 3/PTX3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 5%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 10.4%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    22.3 pg/ml

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102 100% - 105%
    Serum 103 95% - 111%
    Hep Plasma 106 105% - 108%
    EDTA Plasma 113 108% - 120%
    Cit plasma 108 106% - 110%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse PTX3 ELISA Kit (ab245713) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PTX3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PTX3 with 22.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse PTX3 ELISA Kit (ab245713)
    Other - Mouse PTX3 ELISA Kit (ab245713)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse PTX3 Standard Curve Comparison.
    Mouse PTX3 Standard Curve Comparison.
    Standard Curve comparison between mouse PTX3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • Example of mouse PTX3 standard curve in Sample Diluent NS.
    Example of mouse PTX3 standard curve in Sample Diluent NS.
    The PTX3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native PTX3 in mouse serum, plasma, and cell culture supernatant samples.
    Interpolated concentrations of native PTX3 in mouse serum, plasma, and cell culture supernatant samples.
    The concentrations of PTX3 were measured in duplicates, interpolated from the PTX3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and 3T3-L1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTX3 concentration was determined to be 36.40 ng/mL in serum, 33.63 ng/mL in plasma (citrate), 50.51 ng/mL in plasma (EDTA), 51.03 ng/mL in plasma (heparin), and 8.37 ng/mL in 3T3-L1 cell culture supernatant.
  • Sandwich ELISA - Mouse PTX3 ELISA Kit (ab245713)
    Sandwich ELISA - Mouse PTX3 ELISA Kit (ab245713)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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