Anti-OLFM4 antibody (ab85046)
Key features and details
- Rabbit polyclonal to OLFM4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OLFM4 antibody
See all OLFM4 primary antibodies -
Description
Rabbit polyclonal to OLFM4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab98247) -
Positive control
- In Western Blot, ab85046 gave a positive signal in Human liver tissue lysate. In IF, this antibody gave a positive signal in T84 cell line.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MouseApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).Target
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Function
An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion. -
Tissue specificity
Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues. -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Domain
The olfactomedin-like domain is involved in the interaction with cadherin. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10562 Human
- Entrez Gene: 380924 Mouse
- Omim: 614061 Human
- SwissProt: Q6UX06 Human
- SwissProt: Q3UZZ4 Mouse
- Unigene: 508113 Human
- Unigene: 26456 Mouse
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Alternative names
- Antiapoptotic protein GW112 antibody
- bA209J19.1 antibody
- G-CSF-stimulated clone 1 protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-OLFM4 antibody (ab85046)
ab85046 stained T84 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85046 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Western blot - Anti-OLFM4 antibody (ab85046)Anti-OLFM4 antibody (ab85046) at 1 µg/ml + Human liver tissue lysate - total protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Additional bands at: 105 kDa (possible non-specific binding), 45 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab85046 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
References (14)
ab85046 has been referenced in 14 publications.
- Ohkuma R et al. High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer. PLoS One 15:e0226707 (2020). PubMed: 31923206
- Wu A et al. Transmissible gastroenteritis virus targets Paneth cells to inhibit the self-renewal and differentiation of Lgr5 intestinal stem cells via Notch signaling. Cell Death Dis 11:40 (2020). PubMed: 31959773
- Zhang P et al. Dissecting the Single-Cell Transcriptome Network Underlying Gastric Premalignant Lesions and Early Gastric Cancer. Cell Rep 27:1934-1947.e5 (2019). PubMed: 31067475
- Shao S et al. Neutrophil exosomes enhance the skin autoinflammation in generalized pustular psoriasis via activating keratinocytes. FASEB J 33:6813-6828 (2019). PubMed: 30811955
- Balbinot C et al. The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms. J Exp Med 215:911-926 (2018). PubMed: 29439001
Images
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Immunocytochemistry/ Immunofluorescence - Anti-OLFM4 antibody (ab85046)
ab85046 stained T84 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85046 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Western blot - Anti-OLFM4 antibody (ab85046)Anti-OLFM4 antibody (ab85046) at 1 µg/ml + Human liver tissue lysate - total protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Additional bands at: 105 kDa (possible non-specific binding), 45 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab85046 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
