Mouse NOV Matched Antibody Pair Kit (ab215403)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
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Product name
Mouse NOV Matched Antibody Pair Kit
See all CCN3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
8.89 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NOV Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse NOV.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab205570. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse NOV Capture Antibody 2 x 50µg 1 x 50µg Mouse NOV Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse NOV Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Immediate-early protein likely to play a role in cell growth regulation. -
Tissue specificity
Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CCN family member 3
- CCN3
- IBP-9
see all -
Database links
- Entrez Gene: 18133 Mouse
- SwissProt: Q64299 Mouse
- Unigene: 5167 Mouse
Images
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.