Mouse NGF R Matched Antibody Pair Kit (ab215075)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.62 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse NGF R Matched Antibody Pair Kit
See all p75 NGF Receptor kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
4.2 pg/ml -
Range
15.62 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NGF R Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse NGF R.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab205569. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse NGF R Capture Antibody 2 x 50µg 1 x 50µg Mouse NGF R Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse NGF R Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD271
- CD271 antigen
- Gp80 LNGFR
see all -
Database links
- Entrez Gene: 18053 Mouse
- SwissProt: Q9Z0W1 Mouse
- Unigene: 283893 Mouse
Images
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Mouse NGF R standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse NGF R Matched Antibody Pair Kit (ab215075)To learn more about the advantages of recombinant antibodies see here.
