Mouse MMP3 Matched Antibody Pair Kit (ab213738)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.2 pg/ml - 2000 pg/ml
Overview
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Product name
Mouse MMP3 Matched Antibody Pair Kit
See all MMP3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.8 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MMP3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MMP3.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab203363. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse MMP3 Capture Antibody 2 x 50µg 1 x 50µg Mouse MMP3 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse MMP3 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all -
Database links
- Entrez Gene: 17392 Mouse
- SwissProt: P28862 Mouse
- Unigene: 4993 Mouse
Images
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Mouse MMP3 standard curve.
Standard calibration curve. Background subtracted values are graphed. Data provided for demonstration purposes only.
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Sandwich ELISA - Mouse MMP3 Matched Antibody Pair Kit (ab213738)To learn more about the advantages of recombinant antibodies see here.
