Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse MMP3 ELISA Kit (ab203363)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 4.94 pg/ml
  • Range: 18.8 pg/ml - 1200 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse MMP3 ELISA Kit
    See all MMP3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.8%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    4.94 pg/ml

  • Range

    18.8 pg/ml - 1200 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103 99% - 109%
    Serum 112 101% - 126%
    Hep Plasma 103 97% - 110%
    Cit plasma 105 97% - 115%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Pig

  • Product overview

    Mouse MMP3 ELISA Kit (ab203363) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP3 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP3 with 4.94 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Matrix metalloproteinase-3 (MMP3) is a member of the matrix metalloproteinase family, which are involved in breakdown of the extracellular matrix. MMP3 is secreted in a proenzyme form from a variety of cells, including macrophages, osteoblasts, keratinocytes, chondrocytes, fibroblasts and vascular smooth muscle cells. It degrades collagen types II, III, IV, IX and X, along with proteoglycans, elastin, laminin and fibronectin. In addition to normal physiological processes like development, reproduction and wound healing, MMP3 is implicated in humans in the progression of atherosclerosis and tumor initiation.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse MMP3 ELISA Kit (ab203363)
    Other - Mouse MMP3 ELISA Kit (ab203363)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse MMP3 standard curve comparison data.
    Mouse MMP3 standard curve comparison data.

    Standard curve comparison between mouse MMP3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 10-fold increase in sensitivity.

  • Example of MMP3 standard curve.
    Example of MMP3 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of MMP3 in mouse plasma (heparin and citrate) and serum.
    Interpolated concentrations of MMP3 in mouse plasma (heparin and citrate) and serum.

    The concentrations of MMP3 were measured in duplicate and interpolated from the MMP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse MMP3 ELISA Kit (ab203363)
    Sandwich ELISA - Mouse MMP3 ELISA Kit (ab203363)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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