Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse MASPIN ELISA Kit (ab238270)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 18.2 pg/ml
  • Range: 46.88 pg/ml - 3000 pg/ml
  • Sample type: Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse MASPIN ELISA Kit
    See all MASPIN kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Skin extract 8 6.6%

  • Sample type

    Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    18.2 pg/ml

  • Range

    46.88 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 98 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse MASPIN ELISA Kit (ab238270) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MASPIN protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MASPIN with 18.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse MASPIN Capture Antibody 1 x 600µl
    10X Mouse MASPIN Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse MASPIN Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

  • Tissue specificity

    Normal mammary epithelial cells.

  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Alternative names

    • Maspin
    • Ovalbumin
    • Peptidase inhibitor 5
    • PI-5
    • PI5
    • Protease inhibitor 5
    • protease inhibitor 5 (maspin)
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
    • Serpin B5
    • serpin family B member 5
    • Serpin peptidase inhibitor
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5
    • Serpinb5
    • SPB5_HUMAN
    • Spi5
    • Spi7
    see all

  • Database links

    Images

    • Other - Mouse MASPIN ELISA Kit (ab238270)
      Other - Mouse MASPIN ELISA Kit (ab238270)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse MASPIN standard curve in Sample Diluent NS.
      Example of mouse MASPIN standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native MASPIN in mouse skin extract and mouse small intestine extract based on a 5 µg/mL and 1,500 µg/mL extract load, respectively.
      Interpolated concentrations of native MASPIN in mouse skin extract and mouse small intestine extract based on a 5 µg/mL and 1,500 µg/mL extract load, respectively.
      The concentrations of MASPIN were measured in duplicate and interpolated from the MASPIN standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 1,060.3 pg/mL in mouse skin extract and 2,199.6 pg/mL in mouse small intestine extract.
    • Sandwich ELISA - Mouse MASPIN ELISA Kit (ab238270)
      Sandwich ELISA - Mouse MASPIN ELISA Kit (ab238270)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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