Mouse Liver-FABP Matched Antibody Pair Kit (ab220137)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 1.95 pg/ml - 125 pg/ml
Overview
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Product name
Mouse Liver-FABP Matched Antibody Pair Kit
See all liver FABP kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.6 pg/ml -
Range
1.95 pg/ml - 125 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Liver-FABP Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Liver-FABP.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab218262. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Liver-FABP Capture Antibody 2 x 50µg 1 x 50µg Mouse Liver-FABP Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Liver-FABP Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- FABP 1
- FABP1
- FABPL
see all -
Database links
- Entrez Gene: 14080 Mouse
- SwissProt: P12710 Mouse
- Unigene: 22126 Mouse
Images
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Mouse Liver-FABP standard curve.
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Sandwich ELISA - Mouse Liver-FABP Matched Antibody Pair Kit (ab220137)To learn more about the advantages of recombinant antibodies see here.
