Glutamate Assay Kit (Fluorometric) (ab252893)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell culture extracts, Cerebral Spinal Fluid, Serum, Tissue Homogenate
Overview
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Product name
Glutamate Assay Kit (Fluorometric)
See all Glutamate kits -
Detection method
Fluorescent -
Sample type
Serum, Cell culture extracts, Tissue Homogenate, Cerebral Spinal Fluid -
Assay type
Quantitative -
Product overview
Glutamate Assay Kit (Fluorometric) (ab252893) allows for quantification of Glutamate in biological fluids and tissues. The assay is based on an enzymatic reaction in which a fluorogenic probe is reduced producing a stable signal. The reduced fluorophore produces a strong signal (Ex/Em= 535/587 nm), which is directly proportional to the amount of Glutamate in samples. The assay is simple, reproducible, and can specifically detect as low as 5 pmol of Glutamate in a 100 µL reaction.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Glutamate Assay Buffer 1 x 50ml Glutamate Enzyme Mix 1 vial Glutamate Standard (0.1 M) 1 x 0.1ml Glutamate Substrate Mix 1 vial Probe (in DMSO) 1 x 0.4ml -
Research areas
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Relevance
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
Images
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Glutamate standard curve
Data from multiple experiments.
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Measurement of Glutamate.Pooled normal human serum (1:10; 5 µL) and pooled normal human CSF (5 µL; undiluted).
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Measurement of Glutamate amounts.HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates (0.5 µg protein) and mouse muscle extracts (0.5 µg protein).