Mouse Isotyping Lateral Flow Kit (ab190514)
Overview
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Product name
Mouse Isotyping Lateral Flow Kit -
Sample type
Cell culture supernatant, Ascites Fluid, Purified protein -
Assay type
Sandwich (qualitative) -
Assay time
0h 10m -
Assay duration
One step assay -
Product overview
Abcam’s one-step immunochromatographic mouse isotyping kit allows for quick and easy identification of mouse isotypes, subtypes and light chains from a single sample. The sample used can be purified antibody, cell culture supernatant or ascites fluid. For purified antibody and cell culture supernatant the test can also be used to determine the relative purity of a hybridoma cell line. This product is for research use only, not for use in diagnostic applications.
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Notes
Lateral-flow membranes are impregnated with separate lines containing antibodies specific for unique amino acid sequences contained within IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, kappa light chains and lambda light chains. When the isotyping strips are added to a test tube containing a diluted mouse immunoglobulin sample, colloidal gold nanoparticles conjugated with antibody form a soluble immune-complex. The complex is apparent in the form of a red band which allows users to make a qualitative determination for their specific sample.
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Tested applications
Suitable for: Othermore details -
Platform
Flow
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 20 tests Isotyping Mouse Antibody coated test strips (Blue) 1 unit Isotyping Mouse Antibody coated test strips (Red) 1 unit Isotyping Mouse Sample Diluent Buffer 1 x 15ml -
Research areas
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Cellular localization
Kappa light chain: Cytoplasmic and Secreted Lambda Light chain: Cytoplasmic IgA: Secreted IgM: Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein. IgG3: Secreted IgG2a: Secreted IgG1: Secreted. Cell membrane; Single-pass membrane protein IgG2b: Secreted
Images
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Blue Strip (kappa, lambda, IgA and IgM)
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Lateral Flow assay summary -
Red Strips (G1, IgG2a, IgG2b and IgG3)