Clostridium tetani toxin 5S IgG plus ELISA kit (ab247204)
Key features and details
- Range: 0.02 IU/ml - 5 IU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Clostridium tetani toxin 5S IgG plus ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% sample 24 1.306% 3.6% sample 24 1.805% 3.46% sample 24 1.591% 5.34% Inter-assay Sample n Mean SD CV% sample 12 0.06% 9.62% sample 12 0.084% 11.33% sample 12 0.658% 13.99% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Range
0.02 IU/ml - 5 IU/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Clostridium tetani toxin 5S IgG plus ELISA kit (ab247204) is designed for the quantitative determination of IgG class antibodies against Clostridium tetani toxin 5S antigens in human serum or plasma (citrate, heparin).
The quantitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Clostridia are spore-forming gram-positive bacteria. The round spores are built at the terminal end which results in the microscope in a “tennis racket” like shape. Clostridium tetani is ubiquitously present in the soil and intestine of humans and animals. Ingestion of bacteria or growth in the intestine of man or animal is without harm. The spores are extremely resistant towards heat and can stay infectious for a long period. The bacteria can get under the skin by even smallest wounds. In Europe tetanus mainly occurs after injuries and sometimes postoperative, whereas in developing countries Tetanus is widely disseminated. The WHO assumes that one million people die because of tetanus worldwide per year.
Tetanus toxin is an excellent immunogen in man - only one antigenic type of toxin. The only effective way to control tetanus is by prophylactic active immunization.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml anti-human IgG HRP conjugate 1 x 20ml Clostridium tetani toxin 5S IgG Control 1 x 2ml Clostridium tetani toxin Coated Microplate (IgG) 1 unit Clostridium tetani toxin IgG Standard A 1 x 2ml Clostridium tetani toxin IgG Standard B 1 x 2ml Clostridium tetani toxin IgG Standard C 1 x 2ml Clostridium tetani toxin IgG Standard D 1 x 2ml Clostridium tetani toxin IgG Standard E 1 x 2ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Typical Standard Curve – Representative standard curve using ab247204 using the point to point function.