Mouse IL36 gamma/IL-1F9 ELISA Kit (ab277450)
Key features and details
- Sensitivity: 0.35 ng/ml
- Range: 0.328 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL36 gamma/IL-1F9 ELISA Kit
See all IL36 gamma/IL-1F9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.35 ng/ml -
Range
0.328 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107.1 73% - 140% Plasma 115.3 86% - 148% Cell culture media 131.8 118% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL36 gamma/IL-1F9 ELISA Kit (ab277450) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL36 gamma/IL-1F9 in serum (Mouse gamma/IL-1F9 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL36 gamma/IL-1F9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse IL36 gamma/IL-1F9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse IL36 gamma/IL-1F9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse IL36 gamma/IL-1F9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 450X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Mouse IL36 gamma/IL-1F9 Antibody 2 vials Anti-Mouse IL36 gamma IL-1F9 coated Microplate 1 unit Mouse IL36 gamma/IL-1F9 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. -
Tissue specificity
Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 1 epsilon
- IL 1 related protein 2
- IL 1(EPSILON)
see all -
Database links
- Entrez Gene: 215257 Mouse
- SwissProt: Q8R460 Mouse
- Unigene: 249379 Mouse
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.