Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse IL-9 ELISA Kit (ab222505)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.67 pg/ml
  • Range: 3.1 pg/ml - 200 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse IL-9 ELISA Kit
    See all IL-9 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.3%

  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.67 pg/ml

  • Range

    3.1 pg/ml - 200 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97 92% - 101%
    Urine 80 76% - 83%
    Serum 116 110% - 123%
    Hep Plasma 110 98% - 122%
    Cit plasma 110 100% - 127%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse IL-9 ELISA Kit (ab222505) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-9 protein in cit plasma, hep plasma, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-9 with 1.67 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-9 is a cytokine produced by CD4+ helper T cells, and it regulates hematopoietic cells through cell proliferation and the prevention of apoptosis. 

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-9 Capture Antibody 1 x 600µl
    10X Mouse IL-9 Detector Antibody 1 x 600µl
    Mouse IL-9 Lyophilized Recombinant Protein 2 vials
    Antibody Diluent CPR 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit

  • Research areas

  • Function

    Supports IL-2 independent and IL-4 independent growth of helper T-cells.

  • Sequence similarities

    Belongs to the IL-7/IL-9 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Cytokine P40
    • Homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL 9
    • IL-9
    • Il9
    • IL9_HUMAN
    • Interleukin 9
    • Interleukin-9
    • Mast cell growth factor
    • MCGF
    • Megakaryoblast growth factor
    • P40
    • p40 cytokine
    • p40 T cell and mast cell growth factor
    • T cell growth factor 3
    • T cell growth factor p40
    • T-cell growth factor P40
    • TCGF 3
    see all

  • Database links

    Images

    • Other - Mouse IL-9 ELISA Kit (ab222505)
      Other - Mouse IL-9 ELISA Kit (ab222505)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse IL-9 standard curve in Sample Diluent NS.
      Example of mouse IL-9 standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked IL-9 in mouse serum, plasma, urine, and cell culture supernatant samples.
      Interpolated concentrations of spiked IL-9 in mouse serum, plasma, urine, and cell culture supernatant samples.

      The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, urine 25%, and EL4 supernatant 50%.

    • Sandwich ELISA - Mouse IL-9 ELISA Kit (ab222505)
      Sandwich ELISA - Mouse IL-9 ELISA Kit (ab222505)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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