Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human IL-6 R alpha ELISA Kit (ab278117)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 76.3 pg/ml
  • Range: 125 pg/ml - 8000 pg/ml
  • Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-6 R alpha ELISA Kit
    See all IL-6R kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 4 4%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    76.3 pg/ml

  • Range

    125 pg/ml - 8000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103 102% - 104%
    Cell culture media 100 94% - 105%
    Hep Plasma 97 96% - 98%
    EDTA Plasma 97 94% - 99%
    Cit plasma 101 98% - 104%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-6 alpha ELISA kit (ab2778117) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-6 alpha protein in human serum, plasma and cell culture supoernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-6 alpha with 76.3 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Interleukin-6 Receptor (IL-6R, IL-6 Receptor), also known as Interleukin-6 Receptor subunit alpha and membrane glycoprotein 80, is part of a receptor that binds Interleukin-6 (IL-6). IL-6 Receptor weakly binds IL-6 with a low affinity, and then is presented to gp130 to facilitate transition into a high affinity signaling competent hexamer. Activation may lead to regulation of the immune response, acute-phase reactions and hematopoiesis. The production of IL-6 Receptor has been implicated in the pathogenesis of many diseases, including multiple myeloma, auto immune diseases and prostate cancer.IL-6 Receptor has two isoforms. The first is a transmembrane protein in which the N-terminal extracellular portion can be released by proteolytic cleavage. The second isoform is a secreted form that lacks the transmembrane and intracellular portions. This assay is directed at the extracellular portion of IL-6 Receptor and detects both the soluble and membrane bound forms.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Recombinant Antibody benefits
    Recombinant Antibody benefits

    To learn more about the advantages of recombinant antibodies see here.

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    The IL-6 R alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • . Interpolated concentrations of native IL-6 R alpha in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).
    . Interpolated concentrations of native IL-6 R alpha in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).

    The concentrations of IL-6 R alpha were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (EDTA) 20%, plasma (citrate, plasma - heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 34.0 ng/mL in serum, 26.1 ng/mL in plasma (EDTA), 40.8 ng/mL in plasma (citrate), and 33.0 ng/mL in plasma (heparin).

  • Serum from eight individual healthy female donors was measured in duplicate.
    Serum from eight individual healthy female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 31.6 ng/mL with a range of 22.4 – 45.1 ng/mL.

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