Mouse IL-6 ELISA Kit (ab100713)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 600 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-mouse IL-6 (Lyophilized) 2 vials IL-6 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-6 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
Database links
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
Images
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ELISA:IL-6 (Interleukin-6) Mouse ELISA Kit (ab100713)
IL-6 measured in mouse tissue lysates prepared with RIPA buffer and sonicated in an ice-cold water bath for 12 intervals of a total of 3.5 minutes. 0.02-1 mg protein x mL-1 were tested, results shown as IL-6 per mg of extracted protein (duplicates +/- SD).
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ELISA:IL-6 (Interleukin-6) Mouse ELISA Kit (ab100713)IL-6 measured in RIPA lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. Samples were diluted and tested in the range of 1e4-5e5 cells x mL-1, results expressed per million cells; duplicates +/- SD).
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ELISA:IL-6 (Interleukin-6) Mouse ELISA Kit (ab100713)Dilution curves of mouse IL-6 (ab100713) and NIBSC standard (93/730). One pg of standard mouse IL-6 corresponds to 0.37 (+/- 0.07) AU. Background signal subtracted (duplicates; +/- SD).
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ELISA:IL-6 (Interleukin-6) Mouse ELISA Kit (ab100713)Standard curve of mouse IL-6 with background signal subtracted (duplicates; +/- SD).
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Typical standard curveRepresentative standard curve using ab100713
