Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)
![Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)](https://www.abcam.com/ps/products/251/ab251094/Images/ab251094-407786-anti-cytosolic-phospholipase-a2-phospho-s505-antibody-epr18552-bsa-and-azide-free-western-blot.jpg "Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18552] to Cytosolic Phospholipase A2 (phospho S505) - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit monoclonal [EPR18552] to Cytosolic Phospholipase A2 (phospho S505) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251094 is the carrier-free version of ab191605. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251094 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18552 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)All lanes : Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605) at 1/2000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 20 ng/ml TNF-alpha for 10 minutes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab191605, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)](https://www.abcam.com/ps/products/251/ab251094/Images/ab251094-406407-anti-cytosolic-phospholipase-a2-phospho-s505-antibody-epr18552-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)This data was developed using ab191605, the same antibody clone in a different buffer formulation.
ab191605 at 1/50 dilution immunoprecipitating Cytosolic Phospholipase A2 (phospho S505) in NIH/3T3 (mouse embryo) whole cell lysate.
Lane 1 (input): NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate 10µg
Lane 2 (+): ab191605 + NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab191605 in NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate
For western blotting, ab191605 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)](https://www.abcam.com/ps/products/251/ab251094/Images/ab251094-1-anti-cytosolic-phospholipase-a2-phospho-s505-antibody-epr18552-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)This data was developed using ab191605, the same antibody clone in a different buffer formulation. Dot blot analysis of Cytosolic Phospholipase A2 (phospho S505) phospho peptide (Lane 1) and Phospholipase A2 Non-phospho peptide (Lane 2) labeled using ab191605 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes. -
![Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)](https://www.abcam.com/ps/products/251/ab251094/Images/ab251094-4-benefits-of-recombinant-antibodies.png)
Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] - BSA and Azide free (ab251094)