Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7691] to M6PR (cation dependent)
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-M6PR (cation dependent) antibody [EPR7691]
    See all M6PR (cation dependent) primary antibodies

  • Description

    Rabbit monoclonal [EPR7691] to M6PR (cation dependent)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human M6PR (cation dependent) aa 250 to the C-terminus (C terminal).
    Database link: P20645

  • Positive control

    • WB: HAP1, A549, and Human uterus lysates ICC/IF: HeLa cell lysate Flow Cyt: A549 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: M6PR knockout HAP1 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 0.8 µg/ml (purified)

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Rat kidney lysates
    Lane 4 : Rat spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 31 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Flow Cytometry - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    All lanes : Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 1/1000 dilution (Unpurified)

    Lane 1 : A549 lysates
    Lane 2 : Human uterus lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)
    Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

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