Mouse IL-22 ELISA Kit (ab223857)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.15 pg/ml
- Range: 3.13 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-22 ELISA Kit
See all IL-22 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 3.8% Inter-assay Sample n Mean SD CV% Serum 3 6.2% -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.15 pg/ml -
Range
3.13 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 104% - 110% Serum 99 98% - 101% Plasma 101 104% - 110% EDTA Plasma 100 98% - 101% Cit plasma 95 94% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse IL-22 ELISA Kit (ab223857) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-22 protein in cit plasma, edta plasma, plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-22 with 0.15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-22 Capture Antibody 1 x 600µl 10X Mouse IL-22 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IL-22 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that contributes to the inflammatory response in vivo. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto18
- IL 10 related T cell derived inducible factor
- IL 21
see all -
Database links
- Entrez Gene: 50929 Mouse
- SwissProt: Q9JJY9 Mouse
- Unigene: 103585 Mouse
Images
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Other - Mouse IL-22 ELISA Kit (ab223857)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse IL-22 standard curve comparison dataStandard curve comparison between mouse IL-22 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of Mouse IL-22 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-22 in Mouse serum and plasma samplesThe concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 117 pg/mL in serum, 101 pg/mL in plasma (citrate), 193 pg/mL in plasma (heparin), and 137 pg/mL in plasma (EDTA).
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Interpolated concentrations of native IL-22 in stimulated EL4.IL2 and spleen cell culture supernatantThe concentrations of IL-22 were measured in duplicate and interpolated from the IL-22 standard curve and corrected for sample dilution. Undiluted samples are as follows: EL4.IL2 supernatant 25% and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 500 pg/mL in EL4.IL2 supernatant and 225 pg/mL in spleen supernatant.
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Comparison of IL-22 in unstimulated and stimulated cell culture supernatantsEL4.IL2 cells were cultured to semi-confluency and then treated for 48 hours with either 10 µg/mL PHA + 10 ng/mL PMA or DMSO (mock). Rat spleen cells were cultured in the presence or absence of 100 ng/mL IFN-gamma + 1 µg/mL LPS for 4 days. The concentrations of IL-22 were measured in five different dilutions of the supernatant samples in duplicates and interpolated from the IL-22 standard curve. The interpolated values are plotted (mean +/- SD, n=5) in pg per mL of neat supernatant. The mean IL-22 concentration was determined to be 500 pg/mL in stimulated EL4.IL2 supernatant and undetectable in unstimulated EL4.IL2 supernatant. The mean concentration was determined to be 225 pg/mL in stimulated spleen supernatant and 1.7 pg/mL in unstimulated spleen supernatant.
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Sandwich ELISA - Mouse IL-22 ELISA Kit (ab223857)To learn more about the advantages of recombinant antibodies see here.
