Mouse IL-2 R alpha ELISA Kit (ab267643)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-2 R alpha ELISA Kit
See all IL-2 Receptor alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
10.24 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96.14 85% - 105% Plasma 82.55 68% - 107% Cell culture media 105.7 92% - 124% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-2 R alpha ELISA Kit is designed for the quantitative determination of IL-2 R alpha in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse IL-2 R alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 R alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 R alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 R alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects mouse IL-2R alpha, less than 0.1% cross-reactivity with human IL-2R alpha is observed.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse IL-2 R alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse IL-2 R alpha Detection Antibody 2 vials Mouse IL-2 R alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Interleukin 2 receptor alpha chain
- CD25
- CD25 antigen
see all -
Database links
- Entrez Gene: 16184 Mouse
- SwissProt: P01590 Mouse
- Unigene: 915 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.