Mouse IL-2 ELISA Kit (ab223588)
Key features and details
- Sensitivity: 3.7 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 4.7% Inter-assay Sample n Mean SD CV% Overall 3 7.6% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.7 pg/ml -
Range
15.625 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88 87% - 89% Cell culture media 117 115% - 120% EDTA Plasma 99 97% - 101% Cit plasma 94 90% - 98% -
Assay time
2h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-2 ELISA Kit (ab223588) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-2 with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states. Complete efficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-2 Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 2 x 20ml 20X Mouse IL-2 Biotin Detector Antibody 20X 1 x 600µl Antibody Diluent 5BR 1 x 6ml Mouse IL-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml Streptavidin-HRP 2 x 100µl TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
Images
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Mouse Il-2 Competitor standard curve comparison
Standard curve comparison between Mouse IL-2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of mouse IL-2 standard curve in Sample Diluent 10BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse IL-2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike mouse IL-2 in mouse serum and plasma samples.The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, and plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native mouse IL-2 in PHA+PMA stimulated EL4.IL2 cell culture supernatant (2 Days) samples.The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated EL4.IL2 cell culture supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 47.25 ng/mL in neat PHA+PMA stimulated EL4.IL2 cell culture supernatant.
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Comparison of IL-2 in unstimulated and PHA+PMA stimulated EL4.IL2 cell supernatants.EL4.IL2 cells were cultured for 2 days in the presence or absence of 10 µg/mL PHA and 10 ng/mL PMA. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3) in ng per mL of neat supernatant. The mean IL-2 concentration was determined to be 47.25 ng/mL in PHA+PMA stimulated EL4.IL2 cell culture supernatant and undetectable in unstimulated EL4.IL2 cell culture supernatant and DMEM media (not shown).
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Sandwich ELISA - Mouse IL-2 ELISA Kit (ab223588)To learn more about the advantages of recombinant antibodies see here.
