Mouse IL-13 ELISA Kit (ab219634)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.2 pg/ml
- Range: 1.95 pg/ml - 125 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-13 ELISA Kit
See all IL-13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 2.2% Inter-assay Sample n Mean SD CV% supernatant 3 1.4% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 pg/ml -
Range
1.95 pg/ml - 125 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 106% - 111% Serum 97 95% - 98% Hep Plasma 104 101% - 108% EDTA Plasma 98 97% - 99% Cit plasma 97 95% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-13 ELISA Kit (ab219634) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-13 with 1.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-13 Capture Antibody 1 x 600µl 10X Mouse IL-13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse IL-13 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Database links
- Entrez Gene: 16163 Mouse
- SwissProt: P20109 Mouse
- Unigene: 1284 Mouse
Images
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Other - Mouse IL-13 ELISA Kit (ab219634)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IL-13 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native IL-13 in mouse serum and plasma samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).
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Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.
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Sandwich ELISA - Mouse IL-13 ELISA Kit (ab219634)To learn more about the advantages of recombinant antibodies see here.
