Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse IKB alpha Matched Antibody Pair Kit (ab217619)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 62.5 pg/ml - 4000 pg/ml

Overview

  • Product name

    Mouse IKB alpha Matched Antibody Pair Kit
    See all IKB alpha kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    9.8 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse IKB alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IKB alpha.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab206308. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse IKB alpha Capture Antibody 2 x 50µg 1 x 50µg
    Mouse IKB alpha Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse IKB alpha Lyophilized Protein 2 vials 1 vial

  • Research areas

  • Function

    Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

  • Involvement in disease

    Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant

  • Sequence similarities

    Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.

  • Post-translational
    modifications

    Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt

  • Alternative names

    • I kappa B alpha
    • I-kappa-B-alpha
    • IkappaBalpha
    • IkB-alpha
    • IKBA
    • IKBA_HUMAN
    • IKBalpha
    • MAD 3
    • MAD3
    • Major histocompatibility complex enhancer-binding protein MAD3
    • NF kappa B inhibitor alpha
    • NF-kappa-B inhibitor alpha
    • NFKBI
    • NFKBIA
    • Nuclear factor of kappa light chain gene enhancer in B cells
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha
    see all

  • Database links

    Images

    • Mouse IKB alpha standard curve.
      Mouse IKB alpha standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Mouse IKB alpha Matched Antibody Pair Kit (ab217619)
      Sandwich ELISA - Mouse IKB alpha Matched Antibody Pair Kit (ab217619)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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