Mouse HGF ELISA Kit (ab223862)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse HGF ELISA Kit
See all HGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.2% Inter-assay Sample n Mean SD CV% Serum 3 4.9% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.4 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 111 109% - 117% Cell culture extracts 104 101% - 107% Tissue Extracts 119 113% - 123% Cell culture media 109 107% - 112% EDTA Plasma 104 100% - 108% Cit plasma 108 105% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse HGF ELISA Kit (ab223862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HGF protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HGF with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Hepatocyte Growth Factor (HGF) is mitogen of parenchymal hepatocytes. HGF also acts as a growth factor and is expressed by a wide variety of tissue and cell types. HGF binds to the HGF- Receptor to help regulate cell growth, cell motility and morphogenesis. Elevated circulating levels of HGF can be measured as prognostic marker of severity of hypertension and heart failure. HGF is secreted as a full length inactive polypeptide, and then is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta chain which dimerize producing the active heterodimeric molecule. Mouse HGF SimpleStep ELISA® (ab223862) is designed to recognize this dimerized protein.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse HGF Capture Antibody 1 x 600µl 10X Mouse HGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse HGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
Involvement in disease
Deafness, autosomal recessive, 39 -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
Database links
- Entrez Gene: 15234 Mouse
- SwissProt: Q08048 Mouse
- Unigene: 267078 Mouse
Images
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Other - Mouse HGF ELISA Kit (ab223862)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse HGF standard curve comparison dataStandard curve comparison between mouse HGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of mouse HGF standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse HGF standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native HGF in mouse serum and plasma samplesThe concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 11,764 pg/mL in serum, 1099 pg/mL in plasma (EDTA), 691 pg/mL in plasma (citrate) and 896 pg/mL in plasma heparin).
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Interpolated concentrations of native HGF in mouse cell and tissue extracts, extracts based on a 4,000 µg/mL extract loadThe concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 7,859 pg/mL in NIH/3T3 cell extract, 2,472 pg/mL in mouse spleen tissue extract and 1,291 pg/mL in mouse kidney tissue extract.
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Interpolated concentrations of native HGF in mouse 3T3-L1 undifferentiated and 3T3-L1 adipocyte differentiated cell extract samples, sample based on a 1,000 µg/mL extract loadMouse 3T3-L1 cells were either undifferentiated (not allowed to reach confluency) or differentiated (cultured for 48 hours in adipocyte differentiation medium). The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 2,636 pg/mL in undifferentiated 3T3-L1 cell extract and 88 pg/mL in adipocyte differentiated 3T3-L1 cell extract.
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Interpolated concentrations of native HGF in rat serum and plasma samplesThe concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (EDTA) 100%, plasma (citrate) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 79,543 pg/mL in serum, 3,151 pg/mL in plasma (EDTA), 2,395 pg/mL in plasma (citrate) and 2,611 pg/mL in plasma heparin).
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Recombinant rat and mouse HGF standard curvesRecombinant rat HGF was prepared at 4 ng/mL and diluted with a 2-fold titration alongside of mouse HGF standard curve, and assayed for cross reactivity. 100% cross-reactivity was observed.
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Sandwich ELISA - Mouse HGF ELISA Kit (ab223862)To learn more about the advantages of recombinant antibodies see here.
