Mouse Hemopexin ELISA Kit (HPX) (ab157716)
Key features and details
- Sensitivity: 4.5092 ng/ml
- Range: 12.5 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Hemopexin ELISA Kit (HPX)
See all Hemopexin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
4.5092 ng/ml -
Range
12.5 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum >=85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Hemopexin (HPX) Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Hemopexin in biological samples of mice.
In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- Hemopexin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemopexin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemopexin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemopexin in the test sample. The quantity of Hemopexin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-mouse Hemopexin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse Hemopexin Calibrator (lyophilized) 1 vial Mouse Hemopexin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
Database links
- Entrez Gene: 15458 Mouse
- SwissProt: Q91X72 Mouse
- Unigene: 3485 Mouse
Images
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Standard curveRepresentative standard curve using ab157716 Hemopexin Mouse ELISA Kit.