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Mouse GDF-15 ELISA Kit (ab216947)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 22.2 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse GDF-15 ELISA Kit
    See all GDF15 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    EDTA plasma 8 5%
    Inter-assay
    Sample n Mean SD CV%
    EDTA plasma 3 5.7%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    22.2 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 76 70% - 82%
    Cell culture media 89 84% - 93%
    Hep Plasma 83 77% - 91%
    EDTA Plasma 82 81% - 84%
    Cit plasma 85 83% - 89%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse GDF-15 ELISA Kit (ab216947) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GDF-15 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GDF-15 with 22.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    The cytokine growth differentiation factor 15 (GDF-15) is one of 15 growth/differentiation factors (GDF-1 to GDF-15), all of which are members of the BMP family of TGF-beta superfamily proteins. These growth/differentiation factors are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. Specifically, mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 158 aa glycosylated prodomain (aa 31-188) and a 115 aa mature region (aa 189-303). Over aa sequence 189-303, mouse GDF-15 shares 97% and 68% aa identity with rat and human GDF-15, respectively.

    In mice, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes. It is also expressed in the epithelium of the small intestine and mammary gland. GDF proteins have a critical role during embryonic development, particularly in the skeletal, nervous, and muscular systems.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse GDF-15 ELISA Kit (ab216947)
    Other - Mouse GDF-15 ELISA Kit (ab216947)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse GDF-15 standard curve.
    Example of mouse GDF-15 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked GDF-15 in mouse serum and plasma samples.
    Interpolated concentrations of spiked GDF-15 in mouse serum and plasma samples.

    The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 4,030 pg/mL in neat serum, 4,261 pg/mL in neat plasma (citrate), 4,385 pg/mL in neat plasma (EDTA), and 3,981 pg/mL in neat plasma (heparin).

  • Interpolated concentrations of native GDF-15 levels in mouse serum and plasma samples.
    Interpolated concentrations of native GDF-15 levels in mouse serum and plasma samples.

    The concentrations of GDF-15 were measured in duplicate, interpolated from the GDF-15 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 837.9 pg/mL in neat serum, 895.2 pg/mL in neat plasma (citrate), 1,079.6 pg/mL in neat plasma (EDTA), and 884.6 pg/mL in neat plasma (heparin).

  • Interpolated concentrations of native GDF-15 levels in pregnant mouse serum, mouse L929 and lung culture media samples.
    Interpolated concentrations of native GDF-15 levels in pregnant mouse serum, mouse L929 and lung culture media samples.

    The concentrations of GDF-15 were measured in duplicate, interpolated from the GDF-15 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant mouse serum 50%, L929 culture media 100%, and lung culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 3,268 pg/mL in neat pregnant mouse serum, 2,223 pg/mL in L929 culture media, and 3,665 pg/mL in lung culture media.

  • Interpolated concentrations of native GDF-15 in unstimulated and stimulated L929 and J774A.1 culture media samples.
    Interpolated concentrations of native GDF-15 in unstimulated and stimulated L929 and J774A.1 culture media samples.

    Samples were loaded neat (100%). The concentrations of GDF-15 were measured in duplicates and interpolated from the GDF-15 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be below the limit of detection in unstimulated L929 culture media, 2,246 pg/mL in stimulated L929 culture media, 193 pg/mL in unstimulated J774A.1 culture media, and 583 pg/mL in stimulated J774A.1 culture media. L929 cells were grown for 72 hours in MEM media containing 10% fetal horse serum and 100 µg/mL Kanamycin (unstimulated) plus 1.5% PHA and 10 ng/mL PMA (stimulated). J774A.1 cells were grown for 72 hours in HGDMEM media containing 10% fetal bovine serum and 100 µg/mL Kanamycin (unstimulated) plus 1.5% PHA and 10 ng/mL PMA (stimulated).

  • Sandwich ELISA - Mouse GDF-15 ELISA Kit (ab216947)
    Sandwich ELISA - Mouse GDF-15 ELISA Kit (ab216947)
    To learn more about the advantages of recombinant antibodies see here.

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