Mouse ECM1 ELISA Kit (ab252368)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse ECM1 ELISA Kit
See all Extracellular matrix protein 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 1.9% Inter-assay Sample n Mean SD CV% serum 3 2.4% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
19 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104 99% - 110% Tissue Extracts 106 102% - 110% Tissue Culture Media 111 108% - 114% Hep Plasma 98 92% - 107% EDTA Plasma 106 99% - 114% Cit plasma 100 88% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse ECM1 ELISA Kit (ab252368) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ECM1 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ECM1 with 19 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ECM1 is a glycoprotein involved in the modulation of bone mineralization and plays a role in wound healing, scarring, and dermal integrity. Mutations of the ECM1 gene is the basis for lipoid proteinosis, a rare dermatologic disorder. ECM1 is currently being investigated for its role in breast cancer metastasis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse ECM1 Capture Antibody 1 x 600µl 10X Mouse ECM1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse ECM1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. -
Tissue specificity
Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. -
Involvement in disease
Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- ECM 1
- Ecm1
- ECM1_HUMAN
see all -
Database links
- Entrez Gene: 13601 Mouse
- SwissProt: Q61508 Mouse
- Unigene: 3433 Mouse
Images
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Other - Mouse ECM1 ELISA Kit (ab252368)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse ECM1 standard curve in Sample Diluent NS.The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse ECM1 standard curve in 1X Cell Extraction Buffer PTR.The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ECM1 in mouse serum, plasma, and supernatant samples.The concentrations of ECM1 were measured in duplicates, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse serum 1:4, mouse plasma (heparin) 1:5, mouse plasma (citrate) 1:5.7, mouse plasma (EDTA) 1:5, and 20% mouse lung supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 11.60 ng/mL in mouse serum, 16.53 ng/mL in mouse plasma (heparin), 19.12 ng/mL in mouse plasma (citrate) , 24.39 ng/mL in mouse plasma (EDTA), and 11.32 ng/mL in mouse lung supernatant.
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Interpolated concentrations of native ECM1 in 100 µg/mL mouse lung extract.The concentrations of ECM1 were measured in duplicate and interpolated from the ECM1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 1.17 ng/mL in mouse lung extract.
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Sandwich ELISA - Mouse ECM1 ELISA Kit (ab252368)To learn more about the advantages of recombinant antibodies see here.
