Mouse E-Cadherin ELISA Kit (ab197751)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 36 pg/ml
- Range: 156.3 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse E-Cadherin ELISA Kit
See all E Cadherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.4% Inter-assay Sample n Mean SD CV% Serum 3 4.9% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
36 pg/ml -
Range
156.3 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103 100% - 107% Cell culture media 106 103% - 107% Cit plasma 103 103% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow -
Product overview
Mouse E-Cadherin ELISA Kit (ab197751) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Cadherin protein in cit plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Cadherin with 36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
E-Cadherin is a member of the Cadherin family of calcium-dependent cell adhesion proteins. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse E-Cadherin Capture Antibody 1 x 600µl 10X Mouse E-Cadherin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse E-Cadherin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. -
Alternative names
- Arc 1
- Cadherin 1
- cadherin 1 type 1 E-cadherin
see all -
Database links
- Entrez Gene: 12550 Mouse
- SwissProt: P09803 Mouse
Images
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Other - Mouse E-Cadherin ELISA Kit (ab197751)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of E-Cadherin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of E-Cadherin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of E-Cadherin in mouse serum and plasma (citrate).The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 364.1 ng/mL in mouse serum and 304.2 ng/mL in mouse plasma (citrate).
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Interpolated concentrations of native E-Cadherin in mouse liver homogenate extract based on a 250 µg/mL extract load.The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 0.42 ng/mL in mouse liver homogenate extract.
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Sandwich ELISA - Mouse E-Cadherin ELISA Kit (ab197751)To learn more about the advantages of recombinant antibodies see here.
