Mouse Cytokeratin 18 ELISA Kit (ab243678)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Cytokeratin 18 ELISA Kit
See all Cytokeratin 18 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 8.2% Inter-assay Sample n Mean SD CV% extract 3 8.3% -
Sample type
Serum, Tissue Extracts, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
27 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 85% - 93% Tissue Extracts 87 79% - 98% Hep Plasma 82 80% - 85% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Cytokeratin 18 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Cytokeratin 18 protein in mouseserum, plasma and tissue extract.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Cytokeratin 18 is a type I cytoskeletal that plays a role in filament reorganization when phosphorylated. When partnered together with Cytokeratin 8, Cytokeratin 18 is involved in IL-6 mediated barrier protection. Mutations within Cytokeratin 18 has been linked to cryptogenic cirrhosis. Cytokeratin 18 can be cleaved by caspase’s generating a caspase cleaved fragment that is a marker of apoptosis and can be used as a biomarker for liver disease. This caspase cleaved Cytokeratin 18 fragment is the provided protein standard for ab243678, which also recognizes the full length total Cytokeratin 18.
Sensitivity:
The calculated minimal detectable dose (MDD) is 27 pg/mL in Sample Diluent NS.
The calculated minimal detectable dose (MDD) is 54 pg/mL in 1XCell Extraction Buffer PTR.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Cytokeratin 18 Capture Antibody 1 x 600µl 10X Mouse Cytokeratin 18 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse Cytokeratin 18 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Alternative names
- Cell proliferation inducing gene 46 protein
- Cell proliferation inducing protein 46
- Cell proliferation-inducing gene 46 protein
see all -
Database links
- Entrez Gene: 16668 Mouse
- SwissProt: P05784 Mouse
- Unigene: 22479 Mouse
Images
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Other - Mouse Cytokeratin 18 ELISA Kit (ab243678)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Cytokeratin 18 standard curve in Sample Diluent NS.The Cytokeratin 18 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Cytokeratin 18 standard curve in 1X Cell Extraction Buffer PTR.The Cytokeratin 18 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cytokeratin 18 in mouse serum and plasma (heparin).The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 25%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 268 pg/mL in serum, 452 pg/mL in plasma (heparin), 332 pg/mL in plasma (EDTA) and 165 pg/mL in plasma (citrate).
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Interpolated concentrations of native Cytokeratin 18 in mouse salivary gland and liver tissue extract based on a 5 µg/mL and 50 µg/mL extract load respectively.The concentrations of Cytokeratin 18 were measured in duplicate and interpolated from the Cytokeratin 18 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 12,131 pg/mL in salivary gland and 1404 pg/mL in liver tissue extracts.
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Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (ab243678)To learn more about the advantages of recombinant antibodies see here.
