Mouse CXCL11 ELISA Kit (I-TAC) (ab204519)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CXCL11 ELISA Kit (I-TAC)
See all CXCL11 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 4.8% Inter-assay Sample n Mean SD CV% Overall 3 4.7% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.4 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 110% - 120% Serum 113 107% - 116% Hep Plasma 90 86% - 92% EDTA Plasma 104 94% - 122% Cit plasma 116 116% - 121% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Sheep -
Product overview
Mouse CXCL11 ELISA Kit (I-TAC) (ab204519) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL11 (I-TAC) protein in cell culture supernatant, plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL11 (I-TAC) with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CXCL11 (I-TAC) is a chemokine for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment and in skin immune responses.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CXLC11 Capture Antibody 1 x 600µl 10X Mouse CXLC11 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse CXCL11 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. -
Tissue specificity
High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- b R1
- b-R1
- Beta-R1
see all -
Database links
- Entrez Gene: 56066 Mouse
- SwissProt: Q9JHH5 Mouse
- Unigene: 131723 Mouse
Images
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Other - Mouse CXCL11 ELISA Kit (I-TAC) (ab204519)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CXCL11 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of mouse CXCL11 in RAW264.7 cell culture supernatant (unstimulated and LPS stimulated) and mouse lung supernatant (stimulated with LPS and PMA).The concentrations of CXCL11 were measured in duplicate and interpolated from the CXCL11 standard curve and corrected for sample dilution.
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Interpolated concentrations of CXCL11 in mouse serum and plasma.Heparin appears to release erythrocyte bound CXCL11, resulting in higher concentration than plasma samples collected with EDTA or sodium citrate.
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Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (ab204519)To learn more about the advantages of recombinant antibodies see here.
