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Mouse CXCL1 Matched Antibody Pair Kit (GRO alpha) (ab213742)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 7.8 pg/ml - 500 pg/ml

Overview

  • Product name

    Mouse CXCL1 Matched Antibody Pair Kit (GRO alpha)
    See all CXCL1/GRO alpha kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    1.1 pg/ml

  • Range

    7.8 pg/ml - 500 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse CXCL1 Matched Antibody Pair Kits (GRO alpha) include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CXCL1 (GRO alpha).


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216951. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse CXCL1 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse CXCL1 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse CXCL1 Lyophilized Protein 2 vials 1 vial

  • Research areas

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.

  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • CINC-1
    • CXCL1
    • Cytokine-induced neutrophil chemoattractant 1
    • Fibroblast secretory protein
    • Fsp
    • Gro
    • Gro 1
    • Gro A
    • GRO protein, alpha
    • GRO-alpha(1-73)
    • GRO-alpha(6-73)
    • Gro1
    • Gro1 oncogene
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • GROA_HUMAN
    • Growth-regulated alpha protein
    • KC
    • KC chemokine, mouse, homolog of
    • Melanoma growth stimulatory activity
    • melanoma growth stimulatory activity alpha
    • Melanoma growth stimulatory activity, alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • N51
    • NAP-3
    • NAP3
    • Neutrophil-activating protein 3
    • Platelet-derived growth factor-inducible protein KC
    • Scyb 1
    • Scyb1
    • Secretory protein N51
    • Small inducible cytokine subfamily B, member 1
    see all

  • Database links

    Images

    • Mouse CXCL1 (GRO alpha) standard curve.
      Mouse CXCL1 (GRO alpha) standard curve.

      Standard calibration curve. Background subtracted values are graphed. Data provided for demonstration purposes only.

       

    • Sandwich ELISA - Mouse CXCL1 Matched Antibody Pair Kit (GRO alpha) (ab213742)
      Sandwich ELISA - Mouse CXCL1 Matched Antibody Pair Kit (GRO alpha) (ab213742)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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