Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.4 pg/ml
- Range: 0.7 pg/ml - 2800 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha)
See all CXCL1/GRO alpha kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 8 6.8% Inter-assay Sample n Mean SD CV% Mouse serum 3 10.1% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 pg/ml -
Range
0.7 pg/ml - 2800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 109% - 110% Serum 91 88% - 94% Cell culture extracts 95 95% - 96% Hep Plasma 79 72% - 90% EDTA Plasma 94 89% - 98% Cit plasma 94 92% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
CXCL1 in vitro CatchPoint® ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is designed for the quantitative measurement of CXCL1 (GRO alpha) protein in mouse serum, plasma, cell culture supernatants, and cell extract samples.
This CatchPoint ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint® ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint® HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
-
Notes
CXCL1 is a member of the CXC family of chemokines. Chemokines play roles in normal and pathological processes including allergic responses, angiogenesis, inflammation, tumor growth and metastasis. Mouse CXCL2 and CXCL3 share 67% and 60% sequence homology with mouse CXCL1, respectively. Additionally, Rat CXCL1 is 89% homologous with mouse CXCL1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 12ml 10X Mouse CXCL1 Capture Antibody 1 x 600µl 10X Mouse CXCL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 2 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 50X Cell Extraction Enhancer Solution 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI 2 x 6ml Mouse CXCL1 Lyophilized Recombinant Protein (ab9906) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
-
Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
Database links
- Entrez Gene: 14825 Mouse
- SwissProt: P12850 Mouse
- Unigene: 21013 Mouse
Images
-
Other - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
CXCL1 (GRO alpha) standard curve in Sample Diluent NS.The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of spiked CXCL1 (GRO alpha) in mouse serum and plasma samples.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 386.84 pg/mL in neat serum, 399.45 pg/mL in neat plasma (citrate), 364.34 pg/mL in neat plasma (EDTA), and 505.80 pg/mL in neat plasma (heparin).
-
Interpolated concentrations of native CXCL1 (GRO alpha) in mouse serum and plasma samples.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 48.56 pg/mL in neat serum, 24.34 pg/mL in neat plasma (citrate), 23.67 pg/mL in neat plasma (EDTA), and 176.56 pg/mL in neat plasma (heparin).
-
Interpolated concentrations of native CXCL1 (GRO alpha) in mouse NIH 3T3 cell culture supernatant sample.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: NIH 3T3 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 472.88 pg/mL in neat mouse NIH 3T3 cell culture supernatant.
-
Interpolated concentrations of native CXCL1 (GRO alpha) in mouse NIH 3T3 cell extract sample based on a 500 µg/mL extract load.The concentrations of CXCL1 (GRO alpha)were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 89.05 pg/mL in mouse NIH 3T3 cell extract sample
-
Sandwich ELISA - Mouse CXCL1 ELISA Kit, Fluorescent (GRO alpha) (ab229426)To learn more about the advantages of recombinant antibodies see here.
