Mouse MCP3 Matched Antibody Pair Kit (ab210889)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 1.56 pg/ml - 100 pg/ml
Overview
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Product name
Mouse MCP3 Matched Antibody Pair Kit
See all MCP3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.58 pg/ml -
Range
1.56 pg/ml - 100 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MCP3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MCP3.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab205571. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse MCP3 Capture Antibody 2 x 50µg 1 x 50µg Mouse MCP3 Detector Antibody 12 x 12.5µg 1 x 12.5µg Mouse MCP3 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 7
- Ccl7
- CCL7_HUMAN
see all -
Database links
- Entrez Gene: 20306 Mouse
- SwissProt: Q03366 Mouse
- Unigene: 341574 Mouse
Images
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Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse MCP3 Matched Antibody Pair Kit (ab210889)To learn more about the advantages of recombinant antibodies see here.
