Mouse MCP3 ELISA Kit, Fluorescent (ab229391)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.56 pg/ml
- Range: 0.78 pg/ml - 1600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MCP3 ELISA Kit, Fluorescent
See all MCP3 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.24% Inter-assay Sample n Mean SD CV% Serum 3 2.58% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.56 pg/ml -
Range
0.78 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 91% - 99% Plasma 85 80% - 87% Cell culture media 89 89% - 91% EDTA Plasma 93 88% - 96% Cit plasma 98 94% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
MCP3 (CCL7) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MCP3 (CCL7) protein in mouse serum, plasma and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
MCP3, also known as CCL7, belongs to the C-C chemokine family. MCP3 attracts monocytes, eosinophils, NK cells, T-lymphocytes, but not neutrophils. The ability of MCP3 to interact with various immune cell types are explained by the molecule’s ability to interact with various chemokine receptors. The chemokines known to interact with MCP3 include CCR1, CCR2, and CCR3. MCP3 also augments monocyte anti-tumor activity, and has been implicated in various studies investigating cancer metastasis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Mouse MCP3 Capture Antibody 1 x 600µl 10X Mouse MCP3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent CPR 1 x 6ml Mouse MCP3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 7
- Ccl7
- CCL7_HUMAN
see all -
Database links
- Entrez Gene: 20306 Mouse
- SwissProt: Q03366 Mouse
- Unigene: 341574 Mouse
Images
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Other - Mouse MCP3 ELISA Kit, Fluorescent (ab229391)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MCP3 (CCL7) standard curve in Sample Diluent NS.The MCP3 (CCL7) standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
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Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.The 1X dilution was 25% for the mouse serum and all of the mouse plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.
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Titration of mouse stimulated (5 µg/mL LPS, 48 hrs (+)) and unstimulated (-) Raw 264.7 supernatant, and unstimulated (-) D10.G4.1 supernatant.A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 3.13% for the stimulated and unstimulated Raw 264.7 supernatants, and 25.0% for the unstimulated D10.G4.1 supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.
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Sandwich ELISA - Mouse MCP3 ELISA Kit, Fluorescent (ab229391)To learn more about the advantages of recombinant antibodies see here.
