Mouse CHIT1 ELISA Kit (ab246542)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.911 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CHIT1 ELISA Kit
See all CHIT1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.5% Inter-assay Sample n Mean SD CV% Serum 3 2.9% -
Sample type
Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.911 pg/ml -
Range
15.625 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102 98% - 106% Tissue Culture Media 105 96% - 115% Hep Plasma 101 96% - 104% EDTA Plasma 99 95% - 103% Cit plasma 91 85% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse CHIT1 ELISA Kit (ab246542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CHIT1 protein in cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CHIT1 with 3.911 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CHIT1 Capture Antibody 1 x 600µl 10X Mouse CHIT1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse CHIT1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. -
Tissue specificity
Detected in spleen. Secreted by cultured macrophages. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Contains 1 chitin-binding type-2 domain. -
Cellular localization
Secreted. Lysosome. A small proportion is lysosomal. - Information by UniProt
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Alternative names
- CHI3
- CHIT
- CHIT1
see all -
Database links
- Entrez Gene: 71884 Mouse
- SwissProt: Q9D7Q1 Mouse
- Unigene: 328268 Mouse
Images
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Other - Mouse CHIT1 ELISA Kit (ab246542)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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. Example of mouse CHIT1 standard curve in Sample Diluent NS.The CHIT1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Interpolated concentrations of native CHIT1 in mouse serum and plasma.The concentrations of CHIT1 were measured in duplicates, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.5%, plasma (citrate) 0.5%, plasma (EDTA) 0.5%, and plasma (heparin) 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 131.15 ng/mL in serum, 128.06 ng/mL in plasma (citrate), 105.87 ng/mL in plasma (EDTA), and 162.76 ng/mL in plasma (heparin).
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Interpolated concentrations of native CHIT1 in mouse tissue culture supernatant samples.The concentrations of CHIT1 were measured in duplicate, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted spleen supernatant is 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 422.68 pg/mL in spleen supernatant.
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Sandwich ELISA - Mouse CHIT1 ELISA Kit (ab246542)To learn more about the advantages of recombinant antibodies see here.
