Human TARC ELISA Kit (CCL17) (ab183366)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 78.5 pg/ml
- Range: 176 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TARC ELISA Kit (CCL17)
See all TARC/CCL17 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HeLa extract 5 4.4% Inter-assay Sample n Mean SD CV% HeLa extract 3 3.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
78.5 pg/ml -
Range
176 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 112 126% - 98% Cell culture media = 93 95% - 91% Hep Plasma = 123 130% - 117% EDTA Plasma = 108 111% - 104% Cit plasma = 97 98% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TARC ELISA Kit (CCL17) (ab183366) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TARC protein in human cell culture supernatants, serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human TARC with 78.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
TARC is a member of the CC chemokine group that is constitutively expressed in the thymus and is transiently produced by a variety of cell types: dendritic cells (DC), endothelial cells, keratinocytes (KC), bronchial epithelial cells and fibroblasts. TARC is a ligand for chemokine receptor CCR4, expressed on Th2 lymphocytes, basophils and natural killer cells. CCL17 binds to CCR4 to induce chemotaxis in T cells.
TARC production appears to be upregulated by TNF-α and IFN-γ synergistically through NFkB and p38 signaling pathways in Human kertinocytes. Cytokines IL-4 and TGF-β1 act to downregulate TNF-α and IFN-γ induced TARC production in Human keratinocytes. TARC (CCL17) and its receptor CCR4 are believed to play important roles in the pathogenesis of the skin diseases Atopic Dermatitis (AD), Bullous pemphigoid (BP) and Mycosis fungoides (MF).
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TARC Capture Antibody 1 x 600µl 10X Human TARC Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TARC Human Lyophilized Recombinant Protein 2 vials TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. -
Tissue specificity
Expressed at high levels in thymus and at low levels in the lung, colon and small intestine. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A-152E5.3
- A152E53
- ABCD 2
see all -
Database links
- Entrez Gene: 6361 Human
- Omim: 601520 Human
- SwissProt: Q92583 Human
- Unigene: 546294 Human
Images
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Other - Human TARC ELISA Kit (CCL17) (ab183366)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of TARC standard curveThe TARC standard curve was prepared using Sample Diluent. Background-subtracted data values (mean +/- SD) are graphed.
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Example of TARC standard curveThe TARC standard curve was prepared using Sample Diluent. Background-subtracted data values (mean +/- SD) are graphed.
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Titration of PBMC Cell Culture Supernatants diluted within the working range of the assayBackground subtracted data from duplicate measurements are plotted. Bar graph denotes quantification of TARC interpolated from standard curve and multiplied by dilution factor.