Cardiolipin Assay Kit (ab241036)
Key features and details
- Detection method: Fluorescent
Overview
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Product name
Cardiolipin Assay Kit -
Detection method
Fluorescent -
Product overview
The Cardiolipin Assay Kit (ab241036) is a fluorometric assay that makes use of a proprietary probe that fluoresces on association with cardiolipin but not with any other lipids such as phosphatidylcholine and sphingomyelin, making it highly specific.
Fluorescence can be recorded at Ex/Em 340/480 nm. The kit includes purified cardiolipin as standard and can detect as low as 0.2 nmol of cardiolipin.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cardiolipin (5 mM) 1 x 20µl CL Assay Buffer 1 x 25ml CL Probe 1 vial
Images
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Cardiolipin standard curve after subtraction of background.Cardiolipin standard curve after subtraction of background.
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Cardiolipin content per mg protein.Cardiolipin content per mg protein in Jurkat cell lysate, Jurkat cell mitochondria, Bovine heart mitochondria and Yeast Mitochondria (S. cerevisiae).
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Specificity of the Cardiolipin Probe.Signal from phosphatidylcholine (PC), phosphatidylserine (PS), sphingomyelin (SM) and cardiolipin (CL) demonstrate the specificity of the Cardiolipin Probe. Known amount of each lipid was added (5 nmol/well). Assay was performed using kit protocol.
