Mouse CD93 ELISA Kit (ab233620)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.4 pg/ml
- Range: 23.4 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD93 ELISA Kit
See all CD93 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 4.2% Inter-assay Sample n Mean SD CV% Serum 3 5.7% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
3.4 pg/ml -
Range
23.4 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102 98% - 107% Tissue Extracts 99 97% - 112% Hep Plasma 108 101% - 113% EDTA Plasma 101 98% - 106% Cit plasma 103 99% - 110% Tissue Homogenate 108 102% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse CD93 ELISA Kit (ab233620) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD93 protein in edta plasma, hep plasma, serum, tissue extracts, tissue homogenate, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD93 with 3.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CD93 Capture Antibody 1 x 600µl 10X Mouse CD93 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse CD93 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. -
Tissue specificity
Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 5 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- C1q receptor 1
- C1q/MBL/SPA receptor
- C1qR
see all -
Database links
- Entrez Gene: 17064 Mouse
- SwissProt: O89103 Mouse
- Unigene: 681 Mouse
Images
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Other - Mouse CD93 ELISA Kit (ab233620)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse CD93 Competitor standard curve comparisonStandard curve comparison between Mouse CD93 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse CD93 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CD93 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD93 in mouse serum and plasma samplesThe concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 1,000, plasma (citrate) 1: 500, plasma (heparin) 1: 1,000, and plasma (EDTA) 1: 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 863 ng/mL in serum, 633 ng/mL in plasma (citrate), 799 ng/ml in plasma (heparin), and 468 ng/ml in plasma (EDTA).
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Interpolated concentrations of native CD93 in mouse tissue culture supernatant samplesThe concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse lung supernatant 1: 200 and mouse heart supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 103 ng/mL in mouse lung supernatant and 50 ng/ml in mouse heart supernatant.
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Interpolated concentrations of native CD93 in mouse lung, heart, and spleen homogenate extract samples based on a 3 µg/mL, 25 µg/mL, and 50 µg/mL extract load respectivelyThe concentrations of CD93 were measured in duplicate and interpolated from the CD93 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 898 pg/mL in lung homogenate extract, 1116 pg/ml in heart homogenate extract, and 1133 ng/mL in spleen homogenate extract samples.
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Sandwich ELISA - Mouse CD93 ELISA Kit (ab233620)To learn more about the advantages of recombinant antibodies see here.