Mouse CD30-L ELISA Kit (TNFSF8) (ab272476)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.6 pg/ml
- Range: 9.4 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD30-L ELISA Kit (TNFSF8)
See all CD30-L kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.1% Inter-assay Sample n Mean SD CV% Serum 4 9.7% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
6.6 pg/ml -
Range
9.4 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 105% - 107% Serum 103 100% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CD30-L ELISA kit (ab272467) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse CD30-L protein in serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD30-L with 6.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD30-L, also known as CD153 and Tumor Necrosis Factor Ligand Superfamily Member 8 (TNFSF8), is a cytokine that is encoded by the gene TNFSF8. CD30-L is a single-pass type II membrane protein that forms a homotrimer. CD30-L is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma. Engagement of CD30-L expressed on B cell surfaces assists in modulating Ig class switching.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CD30-L (TNFSF8) Capture Antibody 1 x 600µl 10X Mouse CD30-L (TNFSF8) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse CD30-L (TNFSF8) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD153
- CD153 antigen
- CD30 antigen ligand
see all -
Database links
- Entrez Gene: 21949 Mouse
- SwissProt: P32972 Mouse
- Unigene: 4664 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CD30-L standard curve in Sample Diluent NS.The CD30-L standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD30-L in mouse EL4 stimulated and EL4 unstimulated.The concentrations of CD30-L were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: EL4 PHA stimulated 100% and EL4 unstimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 2,331 pg/mL in EL4 stimulated and 111 pg/mL in EL4 unstimulated.
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Interpolated concentrations of native CD30-L in mouse serum.The concentrations of CD30-L were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 571 pg/mL in serum.
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Sandwich ELISA - Mouse CD30-L ELISA Kit (TNFSF8) (ab272476)To learn more about the advantages of recombinant antibodies see here.
