Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse CD30 ELISA Kit (ab264614)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 79 pg/ml
  • Range: 133 pg/ml - 8500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse CD30 ELISA Kit
    See all CD30 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 3.5%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 4 4.4%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    79 pg/ml

  • Range

    133 pg/ml - 8500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 86% - 119%
    Serum 107 98% - 114%
    Hep Plasma 96 93% - 108%
    EDTA Plasma 99 90% - 106%
    Cit plasma 95 87% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse CD30 ELISA Kit (ab264614) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD30 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD30 with 79 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    CD30, also known as Tumor necrosis factor receptor superfamily member 8, is a cell membrane receptor encoded by the gene Tnfrsf8. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CD30 Capture Antibody 1 x 600µl
    10x Mouse CD30 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse CD30 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

  • Sequence similarities

    Contains 6 TNFR-Cys repeats.

  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues.

  • Cellular localization

    Cytoplasm and Cell membrane.
  • Information by UniProt

  • Alternative names

    • CD 30
    • CD30
    • CD30 antigen
    • CD30L receptor
    • Cytokine receptor CD30
    • D1S166E
    • KI 1
    • KI 1 antigen
    • Ki-1 antigen
    • KI1
    • Lymphocyte activation antigen CD30
    • TNFRSF 8
    • Tnfrsf8
    • TNR8_HUMAN
    • Tumor necrosis factor receptor superfamily member 8
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of mouse CD30 standard curve in Sample Diluent NS.
      Example of mouse CD30 standard curve in Sample Diluent NS.

      The CD30 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native CD30 in mouse cell culture supernatant samples.
      Interpolated concentrations of native CD30 in mouse cell culture supernatant samples.

      The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated EL4 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 5.3 ng/mL in stimulated EL4 supernatant.

    • Sandwich ELISA - Mouse CD30 ELISA Kit (ab264614)
      Sandwich ELISA - Mouse CD30 ELISA Kit (ab264614)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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